Are you sure the file is standalone? Downloaded from your site and from that direct link, but both times it gets a "File has stopped working" error whenever I try to open it. Yes, I'm on PC.
Also, the YoYo games plugin crashes when trying to load the game.
It seems most likely that your computer is low on system or graphical memory, then (I know it's a 2D game, but it still uses a respectable amount of memory). Try making sure to close other programs you have open before running it. Also, I've only ever tested it on XP and Vista - I'd be surprised if it didn't work on 2000, but it's possible that if you're using an older version of windows, that could be the cause (I'm running Vista and actually ran into a problem for it on XP - XP had a stricter maximize image size it could parse, so I had to break up some of the tilesets into smaller images).
Anyway, those are my recommendations - the file should definitely work on its own as long as you have enough memory to run it.
*edit* Also, if you get an error message that's a little more descriptive, let me know and I can look into it.